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Significant association between upstream transcription factor 1 rs2516839 polymorphism and hepatocellular carcinoma risk: a case-control study.
Zhao, Xiaodong; Wang, Tianyi; Liu, Bo; Wu, Zhenzhou; Yu, Shuo; Wang, Tao.
Afiliação
  • Zhao X; Department of Oncological Surgery, The Second Hospital of Hebei Medical University, 215 Peace West Road, Shijiazhuang, 050000, China.
Tumour Biol ; 36(4): 2551-8, 2015 Apr.
Article em En | MEDLINE | ID: mdl-25480412
ABSTRACT
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide with high mortality rate. Upstream transcription factor 1 (USF1) is a ubiquitously expressed transcription factor that regulates the expression of genes involving in different biological processes, including cancer. The aim of this study is to examine the correlation between USF1 polymorphisms and HCC susceptibility. Ninety-four HCC patients and 100 healthy volunteers are recruited in our study. Tag single nucleotide polymorphisms (Tag-SNPs) were retrieved in the International HapMap Project Databases. Extraction of genomic DNAs was conducted with TaqMan Blood DNA kits. Genotyping of USF1 polymorphisms were carried out with TaqMan SNPs genotyping assay. Odds ratios (ORs) and their 95% confidence intervals (CIs) were calculated to evaluate the association between USF1 polymorphisms and HCC risk. All statistical analyses were performed with SPSS 20.0 software. Five tag-SNPs were identified to represent the genetic variants of USF1. Our results suggested that rs2516839 in the 5'UTR of USF1 was significantly associated with increased HCC risk (AA vs GG OR = 3.15; 95% CI 1.44-6.87; P = 0.003; GA + AA vs AA OR = 1.85; 95% CI 1.04-3.30; P = 0.034; AA vs GG + GA OR = 2.96; 95% CI 1.40-6.26; P = 0.004; A vs G OR = 2.09; 9% CI 1.35-3.23; P < 0.001). Although rs2073655 in the intron region of USF1 was also shown to be correlated with decreased HCC susceptibility in recessive model (TT vs CC + CT OR = 0.40; 95% CI 0.54-0.75; P = 0.004), this association was not conclusive. Our results indicated that the SNP of rs2516839 have close association with increased risk of HCC. Further studies may be needed to validate our results and gain insights into the pathological mechanism of USF1 gene in the HCC tumorigenesis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Predisposição Genética para Doença / Fatores Estimuladores Upstream / Neoplasias Hepáticas Tipo de estudo: Etiology_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Tumour Biol Assunto da revista: NEOPLASIAS Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Predisposição Genética para Doença / Fatores Estimuladores Upstream / Neoplasias Hepáticas Tipo de estudo: Etiology_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Tumour Biol Assunto da revista: NEOPLASIAS Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China