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Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.
Otto, Oliver; Rosendahl, Philipp; Mietke, Alexander; Golfier, Stefan; Herold, Christoph; Klaue, Daniel; Girardo, Salvatore; Pagliara, Stefano; Ekpenyong, Andrew; Jacobi, Angela; Wobus, Manja; Töpfner, Nicole; Keyser, Ulrich F; Mansfeld, Jörg; Fischer-Friedrich, Elisabeth; Guck, Jochen.
Afiliação
  • Otto O; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Rosendahl P; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Mietke A; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Golfier S; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Herold C; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Klaue D; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Girardo S; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Pagliara S; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Ekpenyong A; 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Department of Physics, Creighton University, Omaha, Nebraska, USA.
  • Jacobi A; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Wobus M; Medizinische Klinik 1, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
  • Töpfner N; Klinik und Poliklinik für Kinder und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
  • Keyser UF; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Mansfeld J; Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
  • Fischer-Friedrich E; 1] Max Planck Institute for the Physics of Complex Systems, Dresden, Germany. [2] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
  • Guck J; 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Cavendish Laboratory, University of Cambridge, Cambridge, UK.
Nat Methods ; 12(3): 199-202, 4 p following 202, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25643151
ABSTRACT
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citometria de Fluxo Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citometria de Fluxo Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha