Flow Cytometry and Electron Microscopy Study of Staphylococcus aureus and Escherichia coli Treated with Mdc-Hly.
Microsc Microanal
; 21(2): 351-7, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25766120
ABSTRACT
In our previous study, a novel hybrid protein combining human lysozyme (Hly) with Musca domestica cecropin (Mdc) was successfully constructed. The broad antibacterial activity against various foodborne pathogens of Mdc-hly suggests its scope as a food preservative. The aim of the present study was to investigate the antibacterial mechanism of the recombinant Mdc-hly. The damage induced by Mdc-hly on Staphylococcus aureus and Escherichia coli was investigated using flow cytometry (FC), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The results of FC showed that Mdc-hly causes bacterial membrane permeabilization. SEM and TEM studies revealed that Mdc-hly is capable of damaging both the membrane and the wall of bacteria, resulting in efflux of essential cytoplasmic contents. Both FC and EM revealed that the effects of Mdc-hly were greater than its parental peptides. Understanding the antibacterial mechanism of Mdc-hly is of a great interest in further utilization of its use in treatment of food and in clinical environments.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
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Microscopia Eletrônica
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Muramidase
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Escherichia coli
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Cecropinas
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Citometria de Fluxo
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Antibacterianos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Microsc Microanal
Ano de publicação:
2015
Tipo de documento:
Article