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Three-dimensional reconstruction of the giant mimivirus particle with an x-ray free-electron laser.
Ekeberg, Tomas; Svenda, Martin; Abergel, Chantal; Maia, Filipe R N C; Seltzer, Virginie; Claverie, Jean-Michel; Hantke, Max; Jönsson, Olof; Nettelblad, Carl; van der Schot, Gijs; Liang, Mengning; DePonte, Daniel P; Barty, Anton; Seibert, M Marvin; Iwan, Bianca; Andersson, Inger; Loh, N Duane; Martin, Andrew V; Chapman, Henry; Bostedt, Christoph; Bozek, John D; Ferguson, Ken R; Krzywinski, Jacek; Epp, Sascha W; Rolles, Daniel; Rudenko, Artem; Hartmann, Robert; Kimmel, Nils; Hajdu, Janos.
Afiliação
  • Ekeberg T; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Svenda M; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Abergel C; Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
  • Maia FR; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Seltzer V; NERSC, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
  • Claverie JM; Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
  • Hantke M; Génomique & Structurale - IGS - UMR 7256, CNRS, Aix-Marseille Université, Institut de Microbiologie de la Méditerranée, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France.
  • Jönsson O; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Nettelblad C; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • van der Schot G; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Liang M; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • DePonte DP; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Barty A; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Seibert MM; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Iwan B; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Andersson I; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Loh ND; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Martin AV; Attophysics Group, CEA-Saclay, 91191 Gif sur Yvette Cedex, France.
  • Chapman H; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
  • Bostedt C; Centre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4 Blk S1 A, Singapore 117546, Singapore.
  • Bozek JD; The University of Melbourne, Parkville, 3010 Victoria, Australia.
  • Ferguson KR; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Krzywinski J; University of Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.
  • Epp SW; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Rolles D; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Rudenko A; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Hartmann R; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Kimmel N; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
  • Hajdu J; Max Planck Advanced Study Group, Center for Free Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
Phys Rev Lett ; 114(9): 098102, 2015 Mar 06.
Article em En | MEDLINE | ID: mdl-25793853
ABSTRACT
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Difração de Raios X / Imageamento Tridimensional / Mimiviridae Idioma: En Revista: Phys Rev Lett Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Suécia
Buscar no Google
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PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Difração de Raios X / Imageamento Tridimensional / Mimiviridae Idioma: En Revista: Phys Rev Lett Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Suécia