Cell cycle-dependent regulation of phosphorylation of the human retinoblastoma gene product.
Science
; 246(4935): 1300-3, 1989 Dec 08.
Article
em En
| MEDLINE
| ID: mdl-2588006
ABSTRACT
The human retinoblastoma gene (RB1) encodes a protein (Rb) of 105 kilodaltons that can be phosphorylated. Analysis of Rb metabolism has shown that the protein has a half-life of more than 10 hours and is synthesized at all phases of the cell cycle. Newly synthesized Rb is not extensively phosphorylated (it is "underphosphorylated") in cells in the G0 and G1 phases but is phosphorylated at multiple sites at the G1/S boundary and in S phase. HL-60 cells that were induced to terminally differentiate by various chemicals lost their ability to phosphorylate newly synthesized Rb at multiple sites when cell growth was arrested. These findings suggest that underphosphorylated Rb may restrict cell proliferation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retinoblastoma
/
Regulação Neoplásica da Expressão Gênica
/
Ciclo Celular
/
Processamento de Proteína Pós-Traducional
/
Proteínas de Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Canadá