Label-free quantitative mass spectrometry for analysis of protein antigens in a meningococcal group B outer membrane vesicle vaccine.
Hum Vaccin Immunother
; 11(6): 1518-25, 2015.
Article
em En
| MEDLINE
| ID: mdl-25997113
Palavras-chave
BCA, bicinchoninic acid; ELISA, enzyme-linked immunosorbent assay; HPLC high performance liquid chromatography; LC-MS/MS, liquid chromatography, tandem mass spectrometry; MRM, multiple reaction monitoring; MS, mass spectrometry; MenB, Meningococcal serogroup B; NadA, Neisseria adhesin A; OMPC, outer membrane protein complex; OMV, outer membrane vesicle; PorA, major outer membrane protein (class I outer membrane protein); QC, quality control; RSD, relative standard deviation; SDS-PAGE, sodium dodecyl sulfate, polyacrylamide gel electrophoresis; WRAIR, Walter Reed Army Institute of Research; fHbp, factor H binding protein; mass spectrometry; membrane proteins; meningococcal group B; multiple reaction monitoring; outer membrane vesicle; protein quantification; vaccine
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Cromatografia Líquida
/
Vacinas Meningocócicas
/
Espectrometria de Massas em Tandem
/
Antígenos de Bactérias
Limite:
Humans
Idioma:
En
Revista:
Hum Vaccin Immunother
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos