Construction of a CRISPR-Cas9 System for Pig Genome Targeting.
Anim Biotechnol
; 26(4): 279-88, 2015.
Article
em En
| MEDLINE
| ID: mdl-26158460
ABSTRACT
A Cas9/sgRNA RNA-guided endonuclease expression system including a codon-optimized Streptococcus pyogenes A20 Cas9 recombinant protein expression vector and a spacer-guide chimeric RNA expression vector using the porcine U6 promoter was constructed for application in pigs. Only the Flag2-NLS1-Cas9-NLS2 recombinant protein in complex with sgRNA was translocated into the nucleus; the Flag2-NLS1-Cas9-NLS2 protein alone was excluded from the nucleus. Up to 13% of porcine PK1 cells targeted in vitro were observed, regardless of transfection efficiency.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Genoma
/
Sus scrofa
/
Sistemas CRISPR-Cas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Anim Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Taiwan