Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt Diet.
Kidney Blood Press Res
; 40(6): 605-13, 2015.
Article
em En
| MEDLINE
| ID: mdl-26619289
ABSTRACT
BACKGROUND/AIMS:
The aim of our study was to investigate the effect of high-salt diet on the renal expression of renalase and the potential role of the local renin-angiotensin system in this process.METHODS:
Sprague-Dawley (SD) rats were divided into groups according to salt content in diet and drug treatment as follows normal-salt diet (NS), high-salt diet (HS), high-salt intake with hydralazine (HS+H), high-salt diet with enalapril (HS+E), and high-salt diet with valsartan (HS+V). The dietary intervention and drugs were given for four weeks. Renin activity and angiotensin II type 1 receptor (AT1R) levels were detected by real-time PCR. Renalase mRNA and protein were also measured.RESULTS:
After four weeks, systolic blood pressure and proteinuria were significantly increased in the HS group with respect to the NS group. Dietary salt intake caused a dramatic decrease in renalase expression in the rat kidneys. Renal cortex renin and AT1R increased significantly in the HS and HS+H groups. Urinary protein was positively correlated with renal renin and AT1R levels. However, in the HS+E and HS+V groups, enalapril and valsartan failed to influence renal renalase expression but abolished the increase in proteinuria, renal cortex renin, and AT1R levels with respect to the HS group.CONCLUSION:
This study indicates that high salt intake reduces renal expression, and renal RAS may be not involved in the regulation of renalase in SD rats fed with high-salt diet.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistema Renina-Angiotensina
/
Cloreto de Sódio na Dieta
/
Rim
/
Monoaminoxidase
Limite:
Animals
Idioma:
En
Revista:
Kidney Blood Press Res
Assunto da revista:
NEFROLOGIA
Ano de publicação:
2015
Tipo de documento:
Article