Uric acid quantification in fingernail of gout patients and healthy volunteers using HPLC-UV.
Biomed Chromatogr
; 30(8): 1338-42, 2016 Aug.
Article
em En
| MEDLINE
| ID: mdl-26787501
ABSTRACT
The presence of elevated uric acid (UA) levels is a sign of gout, that is, hyperuricemia. In this study the monitoring of the UA levels in less-invasive biological samples, such as the human fingernail, is suggested for the diagnosis and therapy of gout. Twenty-six healthy volunteers (HV) and 22 gout patients (GP) were studied. The UA was extracted from human fingernail samples, then separated on an Inertsil ODS-3 column (250 × 4.6 mm i.d., 4.0 µm, GL Sciences) by isocratic elution using methanol-74 mm phosphate buffer (pH 2.2) 298 (v/v). A UV detector was used to monitor the samples at 284 nm. Using the developed method, different UA concentrations were found in the GP and HV. When comparing the concentrations from GP with those from HV, a statistically significant correlation was observed between the UA (p < 0.01). In this study, the UA was confirmed as a potential biomarker for the diagnosis and therapy of gout. We have developed a novel sensitive, and simple method for the determination of UA in the fingernails of GP and HV. The human fingernail may serve as a noninvasive biosample for the diagnosis of gout. Copyright © 2016 John Wiley & Sons, Ltd.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrofotometria Ultravioleta
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Ácido Úrico
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Cromatografia Líquida de Alta Pressão
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Unhas
Tipo de estudo:
Observational_studies
Limite:
Adult
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Aged
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Humans
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Middle aged
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China