Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model.

Amoury, Manal; Kolberg, Katharina; Pham, Anh-Tuan; Hristodorov, Dmitrij; Mladenov, Radoslav; Di Fiore, Stefano; Helfrich, Wijnand; Kiessling, Fabian; Fischer, Rainer; Pardo, Alessa; Thepen, Theophilus; Hussain, Ahmad F; Nachreiner, Thomas; Barth, Stefan

Amoury, Manal; Kolberg, Katharina; Pham, Anh-Tuan; Hristodorov, Dmitrij; Mladenov, Radoslav; Di Fiore, Stefano; Helfrich, Wijnand; Kiessling, Fabian; Fischer, Rainer; Pardo, Alessa; Thepen, Theophilus; Hussain, Ahmad F; Nachreiner, Thomas; Barth, Stefan.

Afiliação

Amoury M; Department of Experimental Medicine and Immunotherapy, Institute for Applied Medical Engineering, University Hospital RWTH Aachen, Pauwelsstr. 20, 52074 Aachen, Germany.
Kolberg K; Department of Experimental Medicine and Immunotherapy, Institute for Applied Medical Engineering, University Hospital RWTH Aachen, Pauwelsstr. 20, 52074 Aachen, Germany; Department of Pharmaceutical Product Development, Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstr.
Pham AT; Department of Experimental Medicine and Immunotherapy, Institute for Applied Medical Engineering, University Hospital RWTH Aachen, Pauwelsstr. 20, 52074 Aachen, Germany.
Hristodorov D; Department of Experimental Medicine and Immunotherapy, Institute for Applied Medical Engineering, University Hospital RWTH Aachen, Pauwelsstr. 20, 52074 Aachen, Germany.
Mladenov R; Department of Experimental Medicine and Immunotherapy, Institute for Applied Medical Engineering, University Hospital RWTH Aachen, Pauwelsstr. 20, 52074 Aachen, Germany.
Di Fiore S; Department of Pharmaceutical Product Development, Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstr. 6, 52074 Aachen, Germany.
Helfrich W; Department of Surgery, Laboratory for Translational Surgical Oncology, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9700 RB Groningen, Netherlands.
Kiessling F; Institute for Experimental Molecular Imaging, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074 Aachen, Germany.
Fischer R; Department of Pharmaceutical Product Development, Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstr. 6, 52074 Aachen, Germany.
Pardo A; Department of Experimental Medicine and Immunotherapy, Institute for Applied Medical Engineering, University Hospital RWTH Aachen, Pauwelsstr. 20, 52074 Aachen, Germany.
Thepen T; Department of Pharmaceutical Product Development, Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstr. 6, 52074 Aachen, Germany.
Hussain AF; Department of Gynecology and Obstetrics, University Hospital RWTH Aachen, Pauwelsstr. 30, 52074 Aachen, Germany.
Nachreiner T; Department of Experimental Medicine and Immunotherapy, Institute for Applied Medical Engineering, University Hospital RWTH Aachen, Pauwelsstr. 20, 52074 Aachen, Germany.
Barth S; South African Research Chair in Cancer Biotechnology, Institute of Infectious Disease and Molecular Medicine (IDM), Department of Integrative Biomedical Sciences, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa. Electronic address: stefan.ba

Cancer Lett ; 372(2): 201-9, 2016 Mar 28.

Article em En | MEDLINE | ID: mdl-26806809

Assuntos

Apoptose/efeitos dos fármacos; Moléculas de Adesão Celular/antagonistas & inibidores; Granzimas/farmacologia; Imunoterapia/métodos; Imunotoxinas/farmacologia; Anticorpos de Cadeia Única/farmacologia; Neoplasias de Mama Triplo Negativas/tratamento farmacológico; Carga Tumoral/efeitos dos fármacos; Animais; Especificidade de Anticorpos; Antígenos de Neoplasias/genética; Antígenos de Neoplasias/imunologia; Antígenos de Neoplasias/metabolismo; Moléculas de Adesão Celular/genética; Moléculas de Adesão Celular/imunologia; Moléculas de Adesão Celular/metabolismo; Linhagem Celular Tumoral; Proliferação de Células/efeitos dos fármacos; Molécula de Adesão da Célula Epitelial; Feminino; Granzimas/genética; Granzimas/farmacocinética; Células HEK293; Humanos; Imunotoxinas/genética; Imunotoxinas/imunologia; Imunotoxinas/farmacocinética; Camundongos Endogâmicos BALB C; Camundongos Nus; Mutação; Proteínas Recombinantes de Fusão/farmacologia; Anticorpos de Cadeia Única/genética; Anticorpos de Cadeia Única/imunologia; Anticorpos de Cadeia Única/farmacocinética; Distribuição Tecidual; Transfecção; Neoplasias de Mama Triplo Negativas/genética; Neoplasias de Mama Triplo Negativas/imunologia; Neoplasias de Mama Triplo Negativas/metabolismo; Neoplasias de Mama Triplo Negativas/patologia; Ensaios Antitumorais Modelo de Xenoenxerto

Palavras-chave

Granzyme B; Human cytolytic fusion protein; Immunotherapy; Medical biotechnology; Triple negative breast cancer

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Moléculas de Adesão Celular / Imunotoxinas / Apoptose / Carga Tumoral / Granzimas / Anticorpos de Cadeia Única / Neoplasias de Mama Triplo Negativas / Imunoterapia Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Cancer Lett Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Alemanha

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