RNA fragments mimicking tRNA analogs interact with cytochrome c.
Mol Biol Rep
; 43(4): 295-304, 2016 Apr.
Article
em En
| MEDLINE
| ID: mdl-26892782
ABSTRACT
In times, when drug seeking assays focus on the natural molecular triggers and their analogs, a deeper insight into molecular mechanisms governing the initial step of intrinsic apoptosis (cytochrome c release) is essential to suppress the immortality of pathologically changed cells. In this study, we examined RNA molecules mimicking mitochondrial tRNAs interacting with cytochrome c and possibly affecting its cellular function. tRNA analogs were designed and synthesized prior to the conformational analysis and gel assays clearly stating the nucleic acid-protein complex formation. The circular dichroism spectroscopic (CD) and microscale thermophoresis examination revealed the structural and conformational differences between four tRNA analogs in their interactions with cytochrome c. Obtained CD spectra and gel studies resulted in the complex ratio estimation and conclusion that not only the complex formation may be preferential towards specific tRNAs present in the cell, but nucleobase modifications are not essential for such interaction.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência
/
Citocromos c
/
Mitocôndrias
/
Conformação de Ácido Nucleico
Limite:
Humans
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Polônia