Site-directed mutagenesis of &#945;-L-rhamnosidase from Alternaria sp. L1 to enhance synthesis yield of reverse hydrolysis based on rational design.

Xu, Li; Liu, Xiaohong; Yin, Zhenhao; Liu, Qian; Lu, Lili; Xiao, Min

Site-directed mutagenesis of α-L-rhamnosidase from Alternaria sp. L1 to enhance synthesis yield of reverse hydrolysis based on rational design.

Xu, Li; Liu, Xiaohong; Yin, Zhenhao; Liu, Qian; Lu, Lili; Xiao, Min.

Afiliação

Xu L; State Key Lab of Microbial Technology and National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, 250100, People's Republic of China.
Liu X; State Key Lab of Microbial Technology and National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, 250100, People's Republic of China.
Yin Z; Qilu Pharmaceutical Co., Ltd., Jinan, 250100, People's Republic of China.
Liu Q; Academy of State Administration of Grain, Beijing, 100037, People's Republic of China.
Lu L; State Key Lab of Microbial Technology and National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, 250100, People's Republic of China.
Xiao M; State Key Lab of Microbial Technology and National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan, 250100, People's Republic of China. minxiao@sdu.edu.cn.

Appl Microbiol Biotechnol ; 100(24): 10385-10394, 2016 Dec.

Article em En | MEDLINE | ID: mdl-27352363

Assuntos

Alternaria/enzimologia; Glicosídeo Hidrolases/metabolismo; Mutagênese Sítio-Dirigida; Proteínas Mutantes/metabolismo; Domínio Catalítico; Glicosídeo Hidrolases/química; Glicosídeo Hidrolases/genética; Glicosídeos/metabolismo; Hidrólise; Modelos Moleculares; Proteínas Mutantes/química; Proteínas Mutantes/genética; Polissacarídeos/metabolismo; Ramnose/metabolismo

Palavras-chave

3D structural modeling; Reverse hydrolysis reaction; Rhamnose-containing chemicals; Rhamnosidase; Site-directed mutagenesis

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mutagênese Sítio-Dirigida / Proteínas Mutantes / Alternaria / Glicosídeo Hidrolases Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2016 Tipo de documento: Article

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