A Combination Strategy for Construction of Peptide-ß2m-H-2K<sup>b</sup> Single Chain with Overlap Extension PCR and One-Step Cloning.

Xu, Tao; Li, Xiaoe; Wu, You; Shahzad, Khawar Ali; Wang, Wei; Zhang, Lei; Shen, Chuanlai

A Combination Strategy for Construction of Peptide-ß2m-H-2K^b Single Chain with Overlap Extension PCR and One-Step Cloning.

Xu, Tao; Li, Xiaoe; Wu, You; Shahzad, Khawar Ali; Wang, Wei; Zhang, Lei; Shen, Chuanlai.

Afiliação

Xu T; Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, P.R. China.
Li X; Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, P.R. China.
Wu Y; Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, P.R. China.
Shahzad KA; Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, P.R. China.
Wang W; Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, P.R. China.
Zhang L; Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, P.R. China.
Shen C; Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing 210009, P.R. China.

J Microbiol Biotechnol ; 26(12): 2184-2191, 2016 Dec 28.

Article em En | MEDLINE | ID: mdl-27586530

Assuntos

Clonagem Molecular/métodos; Epitopos de Linfócito T/genética; Antígenos de Histocompatibilidade Classe I/genética; Peptídeos/genética; Reação em Cadeia da Polimerase/métodos; Animais; Epitopos de Linfócito T/metabolismo; Antígenos de Histocompatibilidade Classe I/metabolismo; Humanos; Camundongos; Peptídeos/metabolismo; Plasmídeos/genética; Plasmídeos/metabolismo

Palavras-chave

MHC tetramer; Overlap extension PCR; antigen-specific T cell; one-step cloning; recombinant gene

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Antígenos de Histocompatibilidade Classe I / Reação em Cadeia da Polimerase / Clonagem Molecular / Epitopos de Linfócito T Tipo de estudo: Evaluation_studies Limite: Animals / Humans Idioma: En Revista: J Microbiol Biotechnol Ano de publicação: 2016 Tipo de documento: Article

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