Determination of propranolol and carvedilol in urine samples using a magnetic polyamide composite and LC-MS/MS.
Bioanalysis
; 8(20): 2115-23, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-27593542
ABSTRACT
AIM:
ß-blockers are compounds that bind with adrenoreceptors hindering their interaction with adrenalin and noradrenalin. They are clinically relevant and they are also used in some sport as doping agents.RESULTS:
A new method based on the combination of dispersive micro-solid phase extraction and LC-MS/MS has been developed to determine propranolol and carvedilol in urine samples. For this purpose a magnetic-polyamide composite is synthesized and used as sorbent. Working under the optimum conditions, the method provides limits of detection and quantification in the range of 0.1-0.15 µg/l and 0.3-0.5 µg/l, for carvedilol and propranolol, respectively. The precision, expressed as RSD, was better than 9.6% and the relative recoveries varied between 73.7 and 81.3%.CONCLUSION:
The methodology is appropriate for the determination of ß-blockers in urine samples at the low microgram per liter range for therapeutic purposes.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Propanolaminas
/
Propranolol
/
Carbazóis
/
Cromatografia Líquida de Alta Pressão
/
Urinálise
/
Antagonistas Adrenérgicos beta
/
Espectrometria de Massas em Tandem
Limite:
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Espanha