Isolation of alpha-linolenic acid from Sutherlandia frutescens and its inhibition of Mycobacterium tuberculosis' shikimate kinase enzyme.
BMC Complement Altern Med
; 16: 366, 2016 Sep 17.
Article
em En
| MEDLINE
| ID: mdl-27639973
ABSTRACT
BACKGROUND:
Sutherlandia frutescens (L) R.Br. is one of traditional herbal medicines that formed the basis of primary health care systems since the earliest days and is still widely used. Sutherlandia is prescribed for people with tuberculosis (TB), but is still not known which compound(s) acts against M. tuberculosis and its mode of action. The aim of this study was to identify and isolate antimycobacterial compounds from S. frutescens extracts against shikimate kinase, a drug target for M. tuberculosis.METHODS:
S. frutescens were dried, ground and extracted with ethanol, dichloromethane methanol and water. Fractionation and separation of compounds was done with column chromatography. Chromatograms were developed in butanol/acetic acid/water (BAW) [2163]; chloroform/methanol/water/formic acid (CMWF1) [601521] and (CMWF2) [21910.3]. Separation and isolation of active compounds were done using preparative HPLC. The activity of the plant extracts were also screened against shikimate kinase enzyme (MtbSK) using the MtbSK inhibition assay.RESULTS:
The DCM MeOH (11) extract showed a high percentage inhibition (with an IC50 of 0.1 µg/ml) of MtbSK and the purified inhibitor was an Alpha-Linolenic Acid (ALA) compound and it had a significant IC50 of 3.7 µg/ml.CONCLUSIONS:
This study demonstrated that ALA from S. frustescens is an inhibitor of shikimate kinase a good drug target for M. tuberculosis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Fosfotransferases (Aceptor do Grupo Álcool)
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Ácido alfa-Linolênico
/
Fabaceae
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Mycobacterium tuberculosis
Idioma:
En
Revista:
BMC Complement Altern Med
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
África do Sul