Effect of terminal 3'-hydroxymethyl modification of an RNA primer on nonenzymatic primer extension.

Pal, Ayan; Das, Rajat S; Zhang, Weicheng; Lang, Megan; McLaughlin, Larry W; Szostak, Jack W

Pal, Ayan; Das, Rajat S; Zhang, Weicheng; Lang, Megan; McLaughlin, Larry W; Szostak, Jack W.

Afiliação

Pal A; Howard Hughes Medical Institute, Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA. szostak@molbio.mgh.harvard.edu and Department of Genetics, Harvard Medical School, 77 Avenue
Das RS; Boston College, Department of Chemistry, Merkert Chemistry Center, 2609 Beacon Street, Chestnut Hill, Massachusetts 02467, USA and Alnylam Pharmaceuticals, 300 Third Street, Cambridge, Massachusetts 02142, USA.
Zhang W; Howard Hughes Medical Institute, Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA. szostak@molbio.mgh.harvard.edu and Department of Chemistry and Chemical Biology, Harvard Univ
Lang M; Boston College, Department of Chemistry, Merkert Chemistry Center, 2609 Beacon Street, Chestnut Hill, Massachusetts 02467, USA.
McLaughlin LW; Boston College, Department of Chemistry, Merkert Chemistry Center, 2609 Beacon Street, Chestnut Hill, Massachusetts 02467, USA.
Szostak JW; Howard Hughes Medical Institute, Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA. szostak@molbio.mgh.harvard.edu and Department of Genetics, Harvard Medical School, 77 Avenue

Chem Commun (Camb) ; 52(80): 11905-11907, 2016 Sep 29.

Article em En | MEDLINE | ID: mdl-27722518

ABSTRACT

ABSTRACT

The significance of the precise position of the hydroxyl at the 3'-end of an RNA primer for nonenzymatic template-directed primer extension is not well understood. We show that an RNA primer terminating in 3'-hydroxymethyl-2',3'-dideoxy-guanosine has greatly diminished activity, suggesting that the spatial preorganization of the terminal sugar contributes significantly to the efficiency of primer extension.

Assuntos

Técnicas de Amplificação de Ácido Nucleico/métodos; RNA/química; Cromatografia Líquida de Alta Pressão; Eletroforese em Gel de Campo Pulsado; Guanosina/química; Espectrometria de Massas; RNA/análise; RNA/síntese química; Estereoisomerismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA / Técnicas de Amplificação de Ácido Nucleico Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2016 Tipo de documento: Article

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