[Interaction of DNAJB13 with HK1 in mouse].
Nan Fang Yi Ke Da Xue Xue Bao
; 36(12): 1684-1688, 2016 Dec 20.
Article
em Zh
| MEDLINE
| ID: mdl-27998865
ABSTRACT
OBJECTIVE:
To investigate the presence of interactions between DNAJB13 and HK1.METHODS:
The open reading frame of Dnajb13 gene was amplified from mouse testis cDNA by PCR. The PCR products were then inserted into pGEX-4T-1 vector after double digestion and identified by sequencing. The recombinant plasmids were transformated into competent DH5a cells, and the fusion protein was expressed with IPTG induction. SDS-PAGE Coomassie brilliant blue staining and Western blot analysis were used to detect the fusion protein expression. The protein precipitated by GST-DNAJB13 in GST pull down assay was detected by Western blotting.RESULTS:
The recombinant plasmid pGEX-4T-1-Dnajb13 was successfully constructed and verified. E.coli transformed with the recombinant plasmid expressed abundant fusion protein. GST pull down assay showed interactions between DNAJB13 and HK1.CONCLUSION:
DNAJB13 interacts with HK1 in mouse testis and probably participates in spermatogenesis and the regulation of sperm motility.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Espermatogênese
/
Proteínas Recombinantes
/
Proteínas de Choque Térmico HSP40
/
Hexoquinase
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Ano de publicação:
2016
Tipo de documento:
Article