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Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential.
Lisov, Alexander V; Belova, Oksana V; Lisova, Zoya A; Vinokurova, Nataliy G; Nagel, Alexey S; Andreeva-Kovalevskaya, Zhanna I; Budarina, Zhanna I; Nagornykh, Maxim O; Zakharova, Marina V; Shadrin, Andrey M; Solonin, Alexander S; Leontievsky, Alexey A.
Afiliação
  • Lisov AV; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia. ssl204@rambler.ru.
  • Belova OV; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Lisova ZA; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Vinokurova NG; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Nagel AS; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Andreeva-Kovalevskaya ZI; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Budarina ZI; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Nagornykh MO; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Zakharova MV; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Shadrin AM; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Solonin AS; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  • Leontievsky AA; Pushchino State Institute of Life Sciences, 3 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
AMB Express ; 7(1): 5, 2017 Dec.
Article em En | MEDLINE | ID: mdl-28050845
ABSTRACT
Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: AMB Express Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Federação Russa
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: AMB Express Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Federação Russa