Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids.

Snijder, Joost; Borst, Andrew J; Dosey, Annie; Walls, Alexandra C; Burrell, Anika; Reddy, Vijay S; Kollman, Justin M; Veesler, David

Snijder, Joost; Borst, Andrew J; Dosey, Annie; Walls, Alexandra C; Burrell, Anika; Reddy, Vijay S; Kollman, Justin M; Veesler, David.

Afiliação

Snijder J; Department of Biochemistry, University of Washington, Seattle, WA, USA.
Borst AJ; Department of Biochemistry, University of Washington, Seattle, WA, USA.
Dosey A; Department of Biochemistry, University of Washington, Seattle, WA, USA.
Walls AC; Department of Biochemistry, University of Washington, Seattle, WA, USA.
Burrell A; Department of Biochemistry, University of Washington, Seattle, WA, USA.
Reddy VS; Department of Integrative Computational and Structural Biology, The Scripps Research Institute, La Jolla, CA, USA.
Kollman JM; Department of Biochemistry, University of Washington, Seattle, WA, USA.
Veesler D; Department of Biochemistry, University of Washington, Seattle, WA, USA. Electronic address: dveesler@uw.edu.

J Struct Biol ; 198(1): 38-42, 2017 04.

Article em En | MEDLINE | ID: mdl-28254381

Assuntos

Microscopia Crioeletrônica/métodos; Vitrificação; Microscopia Crioeletrônica/economia; Congelamento; Substâncias Macromoleculares; Métodos; Manejo de Espécimes

Palavras-chave

Cryo-electron microscopy; Single particle; Vitrification

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia Crioeletrônica / Vitrificação Idioma: En Revista: J Struct Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

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