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Quantification of Cell Turnover in the Bovine Leukemia Virus Model.
de Brogniez, Alix; Barez, Pierre-Yves; Carpentier, Alexandre; Gutierrez, Geronimo; Reichert, Michal; Trono, Karina; Willems, Luc.
Afiliação
  • de Brogniez A; Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium.
  • Barez PY; Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium.
  • Carpentier A; Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium.
  • Gutierrez G; Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, C.C. 1712, Castelar, Argentina.
  • Reichert M; Department of Pathology, National Veterinary Research Institute, Pulawy, Poland.
  • Trono K; Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, C.C. 1712, Castelar, Argentina.
  • Willems L; Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium. Luc.willems@ulg.ac.be.
Methods Mol Biol ; 1582: 173-182, 2017.
Article em En | MEDLINE | ID: mdl-28357670
ABSTRACT
In a perspective of a comparative virology approach, characterization of the bovine leukemia virus (BLV) model may be helpful to better understand infection by the related human T-lymphotropic virus type 1 (HTLV-1). In this paper, we first provide detailed protocols to inoculate cloned BLV proviruses into sheep or cattle. We also describe methods to quantify apoptosis ex vivo and cell turnover in vivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucose Enzoótica Bovina / Vírus da Leucemia Bovina / Modelos Biológicos Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Bélgica

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucose Enzoótica Bovina / Vírus da Leucemia Bovina / Modelos Biológicos Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Bélgica