[Transcription factors NF-kB, HIF-1, HIF-2, growth factor VEGF, VEGFR2 and carboanhydrase IX mRNA and protein level in the development of kidney cancer metastasis].
Mol Biol (Mosk)
; 51(2): 372-377, 2017.
Article
em Ru
| MEDLINE
| ID: mdl-28537244
ABSTRACT
Here, we have investigated the participation of nuclear factors NF-kB, HIF-1 and HIF-2, VEGF, VEGFR2, and carboanhydrase IX in clear-cell renal cancer. We have determined the expression and protein level of transcription factors, VEGF, VEGFR2, and carboanhydrase IX in tumor and normal tissues of 30 patients with kidney cancer. The Real-Time PCR and ELISA were used in the study. The low levels of HIF-1 mRNA expression associated with high levels of HIF-1 protein were also associated with metastasis. The expression levels of VEGF, VEGFR2, and their protein levels are increased in primary tumors of patients with disseminated kidney cancer compared to nonmetastatic cancer. No correlation was revealed between the content of mRNA and encoded proteins in the kidney cancer tissues. The changes in the ratios of mRNA levels and the respective proteins (HIF-1α, HIF-2, NF-kB, VEGF, VEGFR2, and carboanhydrase IX) may contribute to kidney-cancer metastasis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Neoplásica da Expressão Gênica
/
NF-kappa B
/
Receptor 2 de Fatores de Crescimento do Endotélio Vascular
/
Fator A de Crescimento do Endotélio Vascular
/
Fatores de Transcrição Hélice-Alça-Hélice Básicos
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
Neoplasias Renais
/
Proteínas de Neoplasias
/
Proteínas do Tecido Nervoso
Limite:
Female
/
Humans
/
Male
/
Middle aged
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Ano de publicação:
2017
Tipo de documento:
Article