Your browser doesn't support javascript.
loading
Incubation Temperature, But Not Pequi Oil Supplementation, Affects Methane Production, and the Ruminal Microbiota in a Rumen Simulation Technique (Rusitec) System.
Duarte, Andrea C; Holman, Devin B; Alexander, Trevor W; Kiri, Kerstin; Breves, Gerhard; Chaves, Alexandre V.
Afiliação
  • Duarte AC; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, SydneyNSW, Australia.
  • Holman DB; Lethbridge Research Centre, Agriculture and Agri-Food Canada, LethbridgeAB, Canada.
  • Alexander TW; Lethbridge Research Centre, Agriculture and Agri-Food Canada, LethbridgeAB, Canada.
  • Kiri K; Department of Physiology, University of Veterinary MedicineHannover, Germany.
  • Breves G; Department of Physiology, University of Veterinary MedicineHannover, Germany.
  • Chaves AV; School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, SydneyNSW, Australia.
Front Microbiol ; 8: 1076, 2017.
Article em En | MEDLINE | ID: mdl-28701999
ABSTRACT
Lipid supplementation is a promising strategy for methane mitigation in cattle and has been evaluated using several different lipid sources. However, limited studies have assessed the effect of temperature on methane emissions from cattle and changes in incubation temperature have also not been extensively evaluated. The aim of this study was to evaluate the combined effect of pequi oil (high in unsaturated fatty acids) and incubation temperature on fermentation characteristics and microbial communities using the rumen simulation technique. A completely randomized experiment was conducted over a 28-day period using a Rusitec system. The experiment was divided into four periods of 7 days each, the first of which was a 7-day adaptation period followed by three experimental periods. The two treatments consisted of a control diet (no pequi oil inclusion) and a diet supplemented with pequi oil (1.5 mL/day) which increased the dietary fat content to 6% (dry matter, DM-basis). Three fermenter vessels (i.e., replicates) were allocated to each treatment. In the first experimental period, the incubation temperature was maintained at 39°C, decreased to 35°C in the second experimental period and then increased again to 39°C in the third. Pequi oil was continuously supplemented during the experiment. Microbial communities were assessed using high-throughput sequencing of the archaeal and bacterial 16S rRNA gene. Methane production was reduced by 57% following a 4°C decrease in incubation temperature. Supplementation with pequi oil increased the dietary fat content to 6% (DM-basis) but did not affect methane production. Analysis of the microbiota revealed that decreasing incubation temperature to 35°C affected the archaeal and bacterial diversity and richness of liquid-associated microbes, but lipid supplementation did not change microbial diversity.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Clinical_trials Idioma: En Revista: Front Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Clinical_trials Idioma: En Revista: Front Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália