Preparation of wool follicles for proteomic studies.
Anal Biochem
; 539: 8-10, 2017 12 15.
Article
em En
| MEDLINE
| ID: mdl-28860027
ABSTRACT
A variety of techniques were applied to wool follicles stored in William's E culture medium to optimise the extraction of keratin and keratin associated proteins (KAPs). A time course study indicated that the maximum storage time for live skin in this buffer at 20 °C was 24 h, after which degradative loss of protein became significant. Maceration of the skin for 10 min followed by reciprocal action shaking for 14 h had a detrimental effect on keratin extractability. The best approach involved using a Dounce homogeniser as this resulted in the highest amount of Type I and II keratins and KAPs.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lã
/
Proteômica
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2017
Tipo de documento:
Article