Selection of PD1/PD-L1 X-Aptamers.
Biochimie
; 145: 125-130, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-28912094
ABSTRACT
Specific, chemically modified aptamers (X-Aptamers) were identified against two immune checkpoint proteins, recombinant Programmed Death 1 (PD-1) and Programmed Death Ligand 1 (PD-L1). Selections were performed using a bead-based X-Aptamer (XA) library containing several different amino acid functional groups attached to dU at the 5-position. The binding affinities and specificities of the selected XA-PD1 and XA-PDL1 were validated by hPD-1 and hPD-L1 expression cells, as well as by binding to human pancreatic ductal adenocarcinoma tissue. The selected PD1 and PDL1 XAs can mimic antibody functions in in vitro assays.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Adenocarcinoma
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Aptâmeros de Nucleotídeos
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Antígeno B7-H1
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Receptor de Morte Celular Programada 1
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Proteínas de Neoplasias
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochimie
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos