Licochalcone A Inhibits MMPs and ADAMTSs via the NF-κB and Wnt/ß-Catenin Signaling Pathways in Rat Chondrocytes.
Cell Physiol Biochem
; 43(3): 937-944, 2017.
Article
em En
| MEDLINE
| ID: mdl-28957807
ABSTRACT
BACKGROUND:
Osteoarthritis (OA) is a joint disease in which cartilage degradation is the central pathological change. In this study, we investigated the anti-osteoarthritic effects of licochalcone A (Lico A) in rat chondrocytes.METHODS:
Polymerase chain reaction and Western blotting were performed to evaluate the expression of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-5, ADAMTS-4, collagen II, matrix metalloproteinase (MMP)-13 and MMP-1 at both the gene and protein levels, respectively. In addition, the wnt/ß-catenin and nuclear factor kappa B (NF-κB) signaling pathways were also analyzed by Western blotting.RESULTS:
Lico A downregulated ADAMTS-5, ADAMTS-4,MMP-13 and MMP-1 expression, and diminished the IL-1ß-induced inhibition of collagen II. In addition, the activation of ß-catenin and phosphorylation of p65 and IKKα/ß were suppressed by Lico A.CONCLUSIONS:
Our results suggest that Lico A inhibits MMPs and ADAMTS partly via the NF-κB and wnt/ß-catenin signaling pathways in rat chondrocytes. Thus, Lico A may have therapeutic effects in OA.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Metaloendopeptidases
/
NF-kappa B
/
Chalconas
/
Via de Sinalização Wnt
/
Proteínas ADAMTS
Limite:
Animals
Idioma:
En
Revista:
Cell Physiol Biochem
Assunto da revista:
BIOQUIMICA
/
FARMACOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China