Oxidation of Selected Phenothiazine Drugs During Sample Preparation: Effects of Varying Extraction Conditions on the Extent of Oxidation.
J Anal Toxicol
; 42(2): 99-114, 2018 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-29186521
ABSTRACT
Characterization of degradation products formed from selected phenothiazine drugs during standard solid-phase extraction (SPE) approaches is described. An analytical method for promethazine (PMZ), chlorpromazine (CPZ) and their respective N-desmethyl and sulfoxide metabolites in biological samples (bone tissue extract and blood) by ultra performance liquid chromatography-photodiode array detection, using mixed-mode SPE for basic drugs was developed. When ethyl acetateisopropanolammonium hydroxide (80173) was used as the elution solvent during method development, extraneous peaks were observed that were absent in the negative controls. Analysis of extracts of PMZ and CPZ individually showed extraneous peaks, including peaks with retention time and UV spectra suggesting the formation of the sulfoxide metabolites, amongst others. Analytes were then extracted individually and analyzed by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The results confirmed the oxidation of PMZ to its sulfoxide and N-oxide metabolites and oxidation of CPZ to its sulfoxide metabolite. Oxidation was also observed in analysis of whole blood, and thus was not specific to bone tissue extract. To determine if extraction with minimal oxidation was possible, extractions using SPE with a different elution solvent system (dichloromethaneisopropanolammonium hydroxide) and filtration/pass through extraction (FPTE) with and without evaporation were evaluated. The results demonstrated that the sample preparation method highly influenced the extent of oxidation. FPTE without an evaporation step was the only method that did not measurably induce analyte oxidation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenotiazinas
/
Manejo de Espécimes
/
Osso e Ossos
/
Preparações Farmacêuticas
/
Extração em Fase Sólida
/
Extração Líquido-Líquido
Limite:
Humans
Idioma:
En
Revista:
J Anal Toxicol
Ano de publicação:
2018
Tipo de documento:
Article