Diversity of Polyketide Chains Achieved by Deleting the Tailoring Genes in the Biosynthesis of Ansatrienins.
Chembiochem
; 19(3): 256-262, 2018 02 02.
Article
em En
| MEDLINE
| ID: mdl-29193538
ABSTRACT
The ast gene cluster (GenBank accession numbers KF813023.1 and KP284551) was characterized to be responsible for the biosynthesis of ansatrienins in Streptomyces sp. XZQH13, which contains astC, astF1, and astF2 genes involved in the assembly of the N-cyclohexanoyl d-alanyl side chain and the hydroxylation of C-19, respectively. Further to investigating the biosynthetic mechanism of ansatrienins, herein we constructed the mutant strains XZQH13OEΔastF2 and XZQH13OEΔastCΔastF2. Three new ansatrienin analogues, namely, ansatrienolsâ
I-K (1-3), along with trienomycinol (4) and 3-O-demethyltrienomycinol (5), were isolated from the XZQH13OEΔastCΔastF2 strain, and trienomycinâ
A (6) and trienomycinâ
G (7) were isolated from the XZQH13OEΔastF2 strain. Their structures were determined by a combination of high-resolution MS (ESI) and 1D and 2D NMR spectroscopy. Accordingly, a pathway for the biosynthesis of these new ansatrienins was proposed.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Compostos Bicíclicos Heterocíclicos com Pontes
/
Alanina
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Policetídeos
/
Aminofenóis
Idioma:
En
Revista:
Chembiochem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China