The structural gene for a phosphorus-repressible phosphate permease in Neurospora crassa can complement a mutation in positive regulatory gene nuc-1.
Mol Cell Biol
; 8(3): 1376-9, 1988 Mar.
Article
em En
| MEDLINE
| ID: mdl-2966896
ABSTRACT
van+, a gene encoding a phosphorus-repressible phosphate permease, was isolated by its ability to complement nuc-1, a positive regulatory locus that normally regulates van+ expression. This was unexpected because the nuc-1 host already contained a resident van+ gene. Plasmids carrying van+ complemented a nuc-2 mutation as well. Probing of RNA from untransformed wild-type (nuc-1+) and constitutive (nuc-1c) strains by van+ probes indicated that levels of the van+ transcript were subject to control by nuc-1+. Probing of the same RNAs with a cosmid clone, containing approximately 15 kilobases of upstream and downstream DNA, revealed no other detectable phosphorus-regulated transcripts within this 40-kilobase region of the chromosome.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
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Fosfatos
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Genes Reguladores
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Proteínas de Transporte de Fosfato
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Genes
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Neurospora
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Neurospora crassa
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1988
Tipo de documento:
Article