Effect of ultrasonic field on the enzyme activities and ion balance of potential pathogen Saccharomyces cerevisiae.

ABSTRACT

OBJECTIVES: This study aimed at investigating the enzyme activities and ion concentrations in potential pathogen S.cerevisiae upon ultrasonic treatment. METHODS: The activities of ATPase and antioxidase were identified by ATPase, SOD, and CAT assay kits following the instructions. Extracellular Ca2+ and K+ concentrations were determined in an atomic absorption spectrometer with calcium and potassium hollow-cathode lamps as radiation sources. RESULTS: SOD and CAT activities were enhanced by relatively low ultrasonic power at early time points and reduced to lower levels. Total ATPase, Na+/K+-ATPase, and Ca2+/Mg2+-ATPase activities were reduced by ultrasonic field, with higher reducing rate at stronger ultrasonic power and early time points. In addition, ultrasonic field disturbed the Ca2+ and K+ balances in S.cerevisiae cells. CONCLUSIONS: Ultrasonic field resulted in the reduce even the lost of S.cerevisiae cell viability.