A high-throughput method for screening of L-tyrosine high-yield strains by Saccharomyces cerevisiae.
J Gen Appl Microbiol
; 64(4): 198-201, 2018 Sep 27.
Article
em En
| MEDLINE
| ID: mdl-29695662
ABSTRACT
A biosensor screening assay based on the synthesis of betaxanthin was applied to relatively high throughput screening of the L-tyrosine mutant library. In the assays, fluorescence output showed a linear relationship between extracellular L-tyrosine content and yellow pigment formation. In addition, the yellow pigment accumulation of the L-tyrosine high-yield strain can be easily distinguished with the naked eye compared with the wild-type strain. As a result, numerous mutants that exhibited significantly increased coloration, were screened out after random mutagenesis, and p-coumaric acid production in mutants NK-A3 and NK-B4, were remarkably improved by 4-fold more than that of the wild-type strain. In general, this study provides a novel strategy for screening mutant libraries in the search for highly L-tyrosine-producing strains.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Tirosina
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Microbiologia Industrial
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Ensaios de Triagem em Larga Escala
Tipo de estudo:
Diagnostic_studies
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Screening_studies
Idioma:
En
Revista:
J Gen Appl Microbiol
Ano de publicação:
2018
Tipo de documento:
Article