Characterization of recombinant E. coli expressing arsR from Rhodopseudomonas palustris CGA009 that displays highly selective arsenic adsorption.
Appl Microbiol Biotechnol
; 102(14): 6247-6255, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29789881
ABSTRACT
Innovative methods to lower arsenic (As) exposure are sought. The As regulatory protein (ArsR) is reported of having high affinity and specificity to arsenite [As(III)]. Rhodopseudomonas palustris CGA009 is a good model organism for studying As detoxification due to at least three ars operons and four diverse arsRRP1-4 on the genome. In this study, four Escherichia coli harboring arsRRP1-4 derived from CGA009 were engineered and tested regarding their As resistance. The results showed that E. coli (arsRRP2) displayed robust As(III) resistance, and its growth inhibition rate was only 2.9% when exposed to 3.0 mmol/L As(III). At pH 7.0, E. coli (arsRRP2) showed an enhanced As adsorption capacity. As(III) (2.32 mg/g (dry weight, dw)) and 1.47 mg/g arsenate [As(V)] was adsorbed representing a 4.2-fold and 1.3-fold increase respectively compared to the control strain. The adsorption process was well fitted to Langmuir isothermal mode. E. coli (arsRRP2) (1.0~12.0 g/L) could remove 30.3~82.2% of As (III) when exposed to 10 µg/L As(III). No increase in absorption to copper(II), zinc(II), chromium(III), and lead(II) could be detected. Our studies revealed that arsRRP1-4 from CGA009 could confer As(III) resistance; E. coli (arsRRP2) displayed the highest As resistance, selectivity, and adsorption capacity within a wider pH (5.0~9.0) and salinity (0~15.0 g/L NaCl) range, especially important as it could remove As(III) from low concentration As-containing water.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arsênio
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Rodopseudomonas
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Proteínas Recombinantes
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Transativadores
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Farmacorresistência Bacteriana
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Escherichia coli
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China