Utility of formaldehyde fixation for flow cytometry and inactivation of the AIDS associated retrovirus.
J Immunol Methods
; 86(1): 143-9, 1986 Jan 22.
Article
em En
| MEDLINE
| ID: mdl-3003200
ABSTRACT
To maximize safety in the setting of an increasing number of requests for flow cytometric analysis of specimens potentially contaminated with the AIDS retrovirus, we evaluated some commonly used fixatives for their ability to inactivate the infectious potential of the virus. We found that both formaldehyde (0.37% v/v) and paraformaldehyde (0.5% w/v) completely inactivated the infectious activity of both free and cell-associated lymphadenopathy associated virus (LAV), the etiologic agent for the acquired immunodeficiency syndrome (AIDS). Based on encouraging preliminary results we formally evaluated the effect of formaldehyde fixation on flow cytometric parameters. In addition to inactivating LAV, 0.37% formaldehyde in phosphate buffered saline preserved light scatter and fluorescence properties of cells stained with fluorescein isothiocyanate (FITC) and beta-phycoerythrin (PE) conjugated monoclonal antibodies. These findings suggest that formalin fixation may be useful for laboratories performing flow cytometric analysis of specimens potentially contaminated with the AIDS virus.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Deltaretrovirus
/
Citometria de Fluxo
/
Formaldeído
/
Antígenos de Superfície
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1986
Tipo de documento:
Article