[Determination of zearalenone and α-zearalenol in vegetable oil and grain products by C_(18)-Al_2O_3 solid phase extraction column purification coupled with ultra-performance liquid chromatography tandem mass spectrometry].
Wei Sheng Yan Jiu
; 47(4): 615-620, 2018 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-30081990
ABSTRACT
OBJECTIVE:
To develop a method for simultaneous determination of zearalenone( ZEN) and α-zearalenol( α-ZEL) in vegetable oil and grain products by solid phase extraction column purification coupled with ultra-performance liquid chromatography tandem mass spectrometry.METHODS:
Firstly, ZEN and α-ZEL in grain products were extracted by hexane/ethyl acetate( 50 50, V/V), and then extracted as vegetable oil by acetonitrile-water solution( 90 10, V/V), and purified by C_(18)-Al_2O_3 solid phase extraction column. ZEN and α-ZEL was separated by UPLC with acetonitrile-water gradient elution on C_(18) column( 2. 1 mm × 100 mm, 1. 6 µm), and qualified/quantified by mass spectrometry with ESI negative MRM mode with ~(13)C_(18)-zearalenone as internal standard.RESULTS:
The linearity of ZEN and α-ZEL ranged from 1. 0-500 ng/mL. The limit of detection for ZEN and α-ZEL in vegetable oil and grain products was 0. 3 and 0. 2 µg/kg, respectively. The limit of quantification for ZEN and α-ZEL in vegetable oil and grain products was 1. 0 and 0. 5 µg/kg. The average recoveries of ZEN and α-ZEL for spiked samples of 1. 0-100 µg/kg were 93. 5%-108. 0% and 92. 0%-105. 0%. The relative standard deviations of ZEN and α-ZEL were 3. 2%-8. 5% and 4. 6%-7. 8%( n = 6). 55 samples sold in Hangzhou supermarkets were analyzed. ZEN was detected in all corn germ oil with median and maximum contents of 126. 2 and 453. 1 µg/kg. α-ZEL was detected in 50% corn germ oil with median and maximum contents of 2. 0 and 5. 0µg/kg.CONCLUSION:
The method possesses several advantages including sensitivity, precision, good efficiency of purification, simplicity and economy, and it is applicable to the batch analysis of zearalenone and α-zearalenol in vegetable oil and grain products.Palavras-chave
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zearalenona
/
Zeranol
/
Óleos de Plantas
/
Grão Comestível
/
Cromatografia Líquida de Alta Pressão
/
Espectrometria de Massas em Tandem
/
Extração em Fase Sólida
Idioma:
Zh
Revista:
Wei Sheng Yan Jiu
Assunto da revista:
SAUDE PUBLICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China