Lanostane Triterpenes from Gloeophyllum odoratum and Their Anti-Influenza Effects.
Planta Med
; 85(3): 195-202, 2019 Feb.
Article
em En
| MEDLINE
| ID: mdl-30130818
ABSTRACT
In an in vitro screening for anti-influenza agents from European polypores, the fruit body extract of Gloeophyllum odoratum dose-dependently inhibited the cytopathic effect of the H3N2 influenza virus A/Hong Kong/68 (HK/68) in Madin Darby canine kidney cells with a 50% inhibitory concentration (IC50) of 15 µg/mL, a noncytotoxic concentration. After a chromatographic work-up, eight lanostane triterpenes (1 -8 ) were isolated and their structures were elucidated based on high-resolution electrospray ionization mass spectrometry analyses, and one- and two-dimensional nuclear magnetic resonance experiments. Constituents 1 (gloeophyllin K) and 2 (gloeophyllin L) are reported here for the first time, and compounds 5 , 7 , and 8 have not been described for the investigated fungal material so far. The highest activity was determined for trametenolic acid B (3 ) against HK/68 and the 2009 pandemic H1N1 strain A/Jena/8178/09 with IC50 values of 14 and 11 µM, respectively. In a plaque reduction assay, this compound was able to bind to cell-free viruses and to neutralize their infectivity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Triterpenos
/
Basidiomycota
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Vírus da Influenza A Subtipo H1N1
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Vírus da Influenza A Subtipo H3N2
Limite:
Animals
Idioma:
En
Revista:
Planta Med
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Áustria