[Effect of different concentrations of Gegen Qinlian decoction on expression of P-glycoprotein and multi-drug resistance protein transporter in Caco-2 cells].
Zhongguo Zhong Yao Za Zhi
; 43(15): 3135-3139, 2018 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-30200708
ABSTRACT
To investigate the effects of Gegen Qinlian decoction on the expression of P-glycoprotein (P-gp) and multi-drug resistance protein (MRP) in epithelial cells of human colon adenocarcinoma Caco-2 cellsï¼The effects of different concentrations of Gegen Qinlian decoction on the expression levels of p-gp and MRP1-6 mRNA in Caco-2 cells were detected by real time quantitative reverse transcriptase PCR (qRT-PCR).12 h after drug treatment (5.00 g·L⻹), the expression levels of MDR1 and MRP1-6 were significantly down-regulated at concentration of 5.00 g·L⻹; the mRNA expression levels of MDR1ï¼MRP1ï¼MRP2ï¼MRP4ï¼MRP5 and MRP6 were significantly down-regulated at concentration of 2.50 g·L⻹; only the expression levels of MRP2 and MRP5 were significantly affected at concentration of 1.00 g·L⻹. The results showed that the expression levels of MDR1 and MRP1-6 mRNA in Caco-2 cells could be down-regulated in a dose-dependent manner. Gegen Qinlian decoction may reduce drug efflux by down-regulating the mRNA expression of cell transporters in Caco-2 cell, and increase the time of drug action, thereby enhancing the bioavailability of chemotherapeutic drugs.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Proteínas Associadas à Resistência a Múltiplos Medicamentos
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2018
Tipo de documento:
Article