EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas.
Surg Endosc
; 33(2): 658-662, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30374794
ABSTRACT
BACKGROUND:
Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas.METHODS:
Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected.RESULTS:
IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0-1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation.CONCLUSION:
EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pâncreas
/
Ablação por Cateter
/
Endossonografia
/
Cirurgia Assistida por Computador
/
Endoscopia
Limite:
Animals
Idioma:
En
Revista:
Surg Endosc
Assunto da revista:
DIAGNOSTICO POR IMAGEM
/
GASTROENTEROLOGIA
Ano de publicação:
2019
Tipo de documento:
Article