Your browser doesn't support javascript.
loading
Metacaspase involvement in programmed cell death of the marine cyanobacterium Trichodesmium.
Spungin, Dina; Bidle, Kay D; Berman-Frank, Ilana.
Afiliação
  • Spungin D; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
  • Bidle KD; Department of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ, USA.
  • Berman-Frank I; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
Environ Microbiol ; 21(2): 667-681, 2019 02.
Article em En | MEDLINE | ID: mdl-30585394
ABSTRACT
Metacaspases are cysteine specific proteases implicated in cell-signalling, stress acclimation and programmed cell death (PCD) pathways in plants, fungi, protozoa, bacteria and algae. We investigated metacaspase-like gene expression and biochemical activity in the bloom-forming, N2 -fixing, marine cyanobacterium Trichodesmium, which undergoes PCD under low iron and high-light stress. We examined these patterns with respect to in-silico analyses of protein domain architectures that revealed a diverse array of regulatory domains within Trichodesmium metacaspases-like (TeMC) proteins. Experimental manipulations of laboratory cultures and oceanic surface blooms of Trichodesmium from the South Pacific Ocean triggered PCD under Fe-limitation and high light along with enhanced TeMC activity and upregulated expression of diverse TeMC representatives containing different regulatory domains. Furthermore, TeMC activity was significantly and positively correlated with caspase-like activity, which has been routinely observed to increase with PCD induction in Trichodesmium. Although both TeMC and caspase-like activities were stimulated upon PCD induction, inhibitor treatments of these proteolytic activities provided further evidence of largely distinct substrate specificities, even though some inhibitory crossover was observed. Our findings are the first results linking metacaspase expression and activity in PCD induced mortality in Trichodesmium. Yet, the role/s and specific activities of these different proteins remain to be elucidated.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Apoptose / Caspases / Trichodesmium Limite: Animals Idioma: En Revista: Environ Microbiol Assunto da revista: MICROBIOLOGIA / SAUDE AMBIENTAL Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Israel

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Apoptose / Caspases / Trichodesmium Limite: Animals Idioma: En Revista: Environ Microbiol Assunto da revista: MICROBIOLOGIA / SAUDE AMBIENTAL Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Israel