[The variability of response of thrombocytes to ADP: from theory of thrombogenesis to practical application of plasma rich in thrombocytes].

ABSTRACT

The study was carried out to evaluate variability of morpho-genetic potential of plasma rich in thrombocytes. The analysis of ADP-induced aggregation of thrombocytes separated from blood samples of 32 male volunteers aged 18-42 (22±3) was applied. The bimodal character of reaction of thrombocytes to ADP with peaks of aggregation in range of 30-50% and 60-80%. Besides individual differences in range of aggregation differences in time of reaching peak of aggregation and entering plateau were established. In some of volunteers the reversible character of aggregation curve reflecting absence of phase of thrombogenesis stabilization was established. The characteristics of aggregation curve, associated with activation of signal system, launching of degranulation of thrombocytes and exposition of GPIIaIIIb can reflect velocity and effectiveness of secretion of granules of thrombocytes at application of standard doses of stimulants of aggregation. These specifics substantiate necessity of preliminary evaluation of aggregation response of thrombocytes for prognosis of effectiveness of degranulation and release out of granules of thrombocytes biologically active molecules determining morpho-genetic potential of plasma rich with thrombocytes.