A practical workflow for capillary microsampling in nonclinical studies.
Bioanalysis
; 11(3): 175-184, 2019 Feb.
Article
em En
| MEDLINE
| ID: mdl-30767556
ABSTRACT
Aim:
The result of investigation on the procedure of sample handling and bioanalysis of small volume of plasma sample for nonclinical studies stored in 0.5-ml micronic tubes was reported. Results/methodology:
Sample integrity of the small volume (25 µl) during long-term storage and the feasibility and data reliability of performing multiple re-assays on the small volume sample using 5 µl aliquot per analysis was evaluated.Conclusion:
Integrity was maintained in samples (25 µl) stored for up to 1 month in 0.5-ml micronic tubes at -20°C. A 25 µl sample is sufficient for four-times of re-assays. This evaluation demonstrated the feasibility of this workflow of handling and bioanalysis on small volume plasma sample for GLP studies under the US FDA guidance.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos