New support for the large-scale purification of proteins.
J Chromatogr
; 376: 259-67, 1986 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-3086352
ABSTRACT
We propose a new affinity sorbent, matrix-linked histidine, for the large-scale purification of proteins. A variety of proteins and certain peptides, each distinct from the other, were purified. Immunoglobulin G from human placenta was chosen for a detailed study concerning the effects of coupling, spacer-arm and other parameters. Multiple interactions such as charge-transfer and other ionic reactions have been suggested to be responsible for the interactions between proteins and ligand.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Serina Endopeptidases
/
Proteínas
Limite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1986
Tipo de documento:
Article