Efficient expression and secretion of endo-1,4-ß-xylanase from Penicillium citrinum in non-conventional yeast Yarrowia lipolytica directed by the native and the preproLIP2 signal peptides.

Ouephanit, Chanika; Boonvitthya, Nassapat; Theerachat, Monnat; Bozonnet, Sophie; Chulalaksananukul, Warawut

Ouephanit, Chanika; Boonvitthya, Nassapat; Theerachat, Monnat; Bozonnet, Sophie; Chulalaksananukul, Warawut.

Afiliação

Ouephanit C; Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Biofuels by Biocatalysts Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University,
Boonvitthya N; Innovation Institute, PTT Public Co., Ltd., Ayutthaya, 13170, Thailand.
Theerachat M; Biofuels by Biocatalysts Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Bozonnet S; LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, 31077, France.
Chulalaksananukul W; Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Biofuels by Biocatalysts Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address: warawut.c@chula.ac.th.

Protein Expr Purif ; 160: 1-6, 2019 08.

Article em En | MEDLINE | ID: mdl-30923012

Assuntos

Endo-1,4-beta-Xilanases/genética; Proteínas Fúngicas/genética; Penicillium/enzimologia; Yarrowia/genética; Cromatografia por Troca Iônica; Endo-1,4-beta-Xilanases/química; Endo-1,4-beta-Xilanases/isolamento & purificação; Endo-1,4-beta-Xilanases/metabolismo; Estabilidade Enzimática; Proteínas Fúngicas/química; Proteínas Fúngicas/isolamento & purificação; Proteínas Fúngicas/metabolismo; Expressão Gênica; Concentração de Íons de Hidrogênio; Cinética; Penicillium/genética; Sinais Direcionadores de Proteínas; Transporte Proteico; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Yarrowia/metabolismo

Palavras-chave

Expression; Penicillium citrinum, preproLIP2; TEF promoter; Xylanase A; Yarrowia lipolytica

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Penicillium / Proteínas Fúngicas / Yarrowia / Endo-1,4-beta-Xilanases Idioma: En Revista: Protein Expr Purif Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article

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