Large scale, rapid purification of recombinant tissue-type plasminogen activator.
FEBS Lett
; 209(1): 13-7, 1986 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-3100325
ABSTRACT
Recombinant tissue-type plasminogen activator (rt-PA) from cultures of a genetically manipulated Bowes melanoma cell line (TRBM6) was purified in batches of average volume 451 using an autoclavable, reusable, continuous chromatography system comprising zinc chelate-Sepharose CL4B and lysine-Sepharose CL4B. After eight successive purifications the rt-PA was ultrafiltered to yield a preparation containing 4.9 mg protein/ml and 2.7 X 10(6) IU/ml. Analysis by SDS-polyacrylamide gel electrophoresis followed by staining with Coomassie brilliant blue R250 showed major protein bands at Mr = 63,000 and 65,000; most of the material was in the 1-chain form. The potential usefulness of a simple, rapid continuous chromatography system that can be operated under aseptic conditions is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Ativador de Plasminogênio Tecidual
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1986
Tipo de documento:
Article