CRISPR-Cas9 directed genome engineering for enhancing salt stress tolerance in rice.
Semin Cell Dev Biol
; 96: 91-99, 2019 12.
Article
em En
| MEDLINE
| ID: mdl-31075379
ABSTRACT
Crop productivity in rice is harshly limited due to high concentration of salt in the soil. To understand the intricacies of the mechanism it is important to unravel the key pathways operating inside the plant cell. Emerging state-of-the art technologies have provided the tools to discover the key components inside the plant cell for salt tolerance. Among the molecular entities, transcription factors and/or other important components of sensing and signaling cascades have been the attractive targets and the role of NHX and SOS1 transporters amply described. Not only marker assisted programs but also transgenic approaches by using reverse genetic strategies (knockout or knockdown) or overexpression have been extensively used to engineer rice crop. CRISPR/Cas is an attractive paradigm and provides the feasibility for manipulating several genes simultaneously. Here, in this review we highlight some of the molecular entities that could be potentially targeted for generating rice amenable to sustain growth under high salinity conditions by employing CRISPR/Cas. We also try to address key questions for rice salt stress tolerance other than what is already known.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Sistemas CRISPR-Cas
/
Edição de Genes
/
Estresse Salino
Idioma:
En
Revista:
Semin Cell Dev Biol
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article