Activities of Biapenem against Mycobacterium tuberculosis in Macrophages and Mice.
Biomed Environ Sci
; 32(4): 235-241, 2019 Apr.
Article
em En
| MEDLINE
| ID: mdl-31217059
ABSTRACT
OBJECTIVE:
To assess the activities of biapenem against multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis.METHODS:
Biapenem/clavulanate (BP/CL) was evaluated for in vitro activity against Mycobacterium tuberculosis (Mtb) multidrug-resistant (MDR) isolates, extensively drug-resistant (XDR) isolates, and the H37RV strain. BP/CL activity against the H37Rv strain was assessed in liquid cultures, in macrophages, and in mice..RESULTS:
BP/CL exhibited activity against MDR and XDR Mtb isolates in liquid cultures. BP/CL treatment significantly reduced the number of colony forming units (CFU) of Mtb within macrophages compared with control untreated infected macrophages. Notably, BP/CL synergized in pairwise combinations with protionamide, aminosalicylate, and capreomycin to achieve a fractional inhibitory concentration for each pairing of 0.375 in vitro. In a mouse tuberculosis infection model, the efficacy of a cocktail of levofloxacin + pyrazinamide + protionamide + aminosalicylate against Mtb increased when the cocktail was combined with BP/CL, achieving efficacy similar to that of the positive control treatment (isoniazid + rifampin + pyrazinamide) after 2 months of treatment.CONCLUSION:
BP/CL may provide a new option to clinically treat MDR tuberculosis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tienamicinas
/
Tuberculose Resistente a Múltiplos Medicamentos
/
Anti-Infecciosos
/
Mycobacterium tuberculosis
Limite:
Animals
Idioma:
En
Revista:
Biomed Environ Sci
Assunto da revista:
SAUDE AMBIENTAL
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China