Cell-free synthesis of connexin 43-integrated exosome-mimetic nanoparticles for siRNA delivery.
Acta Biomater
; 96: 517-536, 2019 09 15.
Article
em En
| MEDLINE
| ID: mdl-31284098
ABSTRACT
Exosomes are naturally secreted nanovesicles that have emerged as a promising therapeutic nanodelivery platform, due to their specific composition and biological properties. However, challenges like considerable complexity, low isolation yield, drug payload, and potential safety concerns substantially reduce their pharmaceutical acceptability. Given that the nano-bio-interface is a crucial factor for nanocarrier behavior and function, modification of synthetic nanoparticles with the intrinsic hallmarks of exosomes' membrane to create exosome mimetics could allow for siRNA delivery in a safer and more efficient manner. Herein, connexin 43 (Cx43)-embedded, exosome-mimicking lipid bilayers coated chitosan nanoparticles (Cx43/L/CS NPs) were constructed by using cell-free (CF) synthesis systems with plasmids encoding Cx43 in the presence of lipid-coated CS NPs (L/CS NPs). The integration of de novo synthesized Cx43 into the lipid bilayers of L/CS NPs occurred cotranslationally during one-pot reaction and, more importantly, the integrated Cx43 was functionally active in transport. In addition to considerably lower cytotoxicity (Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistemas de Liberação de Medicamentos
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Conexina 43
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RNA Interferente Pequeno
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Materiais Biomiméticos
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Nanopartículas
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Exossomos
Limite:
Humans
Idioma:
En
Revista:
Acta Biomater
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China