Template-switching mechanism of a group II intron-encoded reverse transcriptase and its implications for biological function and RNA-Seq.

Lentzsch, Alfred M; Yao, Jun; Russell, Rick; Lambowitz, Alan M

Lentzsch, Alfred M; Yao, Jun; Russell, Rick; Lambowitz, Alan M.

Afiliação

Lentzsch AM; Institute for Cellular and Molecular Biology, Departments of Molecular Biosciences and Oncology, University of Texas at Austin, Austin, Texas 78712.
Yao J; Institute for Cellular and Molecular Biology, Departments of Molecular Biosciences and Oncology, University of Texas at Austin, Austin, Texas 78712.
Russell R; Institute for Cellular and Molecular Biology, Departments of Molecular Biosciences and Oncology, University of Texas at Austin, Austin, Texas 78712.
Lambowitz AM; Institute for Cellular and Molecular Biology, Departments of Molecular Biosciences and Oncology, University of Texas at Austin, Austin, Texas 78712 lambowitz@austin.utexas.edu.

J Biol Chem ; 294(51): 19764-19784, 2019 12 20.

Article em En | MEDLINE | ID: mdl-31712313

Assuntos

Íntrons; Nucleotídeos/genética; DNA Polimerase Dirigida por RNA/metabolismo; RNA-Seq/métodos; Retroelementos/genética; Moldes Genéticos; Animais; Primers do DNA; Elementos de DNA Transponíveis; Teste de Complementação Genética; Insetos; Cinética; RNA/genética; Retroviridae/genética; Temperatura; Sequenciamento do Exoma

Palavras-chave

DNA sequencing; RNA; RNA sequencing; RNA virus; RNA-dependent RNA polymerase; chemical biology; enzyme kinetics; group II intron reverse transcriptase; non-templated nucleotide addition; retrovirus; reverse transcription; structurefunction; thermostable group II intron reverse transcriptase; transposable element (TE); viral polymerase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Moldes Genéticos / Íntrons / DNA Polimerase Dirigida por RNA / Retroelementos / RNA-Seq / Nucleotídeos Limite: Animals Idioma: En Revista: J Biol Chem Ano de publicação: 2019 Tipo de documento: Article

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