Enzyme-triggered fluorescence turn-off/turn-on of carbon dots for monitoring ß-glucosidase and its inhibitor in living cells.
Luminescence
; 35(2): 222-230, 2020 Mar.
Article
em En
| MEDLINE
| ID: mdl-31713314
ABSTRACT
Energy transfer engineering based on fluorescent probes for directly sensing enzyme activities are in great demand as enzyme-mediated transformations, which are central to all biological processes. Here, a fluorescence carbon dot (CD)-based assay exhibiting selective responses to the quantitation of ß-glucosidase and the effect of its inhibitor was developed. The most common substrate, para-nitrophenyl-ß-d-glucopyranoside (pNPG) was hydrolyzed by ß-glucosidase to release p-nitrophenol (pNP), which can efficiently quench fluorescence of CDs via an inner filter effect and electron transfer. However, in the presence of inhibitors of ß-glucosidase, the fluorescence intensity gradually recovered as the concentration of inhibitors increased. Therefore, the enzyme-triggered fluorescence turn-off/turn-on of specific CDs successfully achieved sensitive detection of ß-glucosidase and monitored the effect of its inhibitors. This new strategy was applied to detect ß-glucosidase and monitor ß-glucosidase inhibitor in hepatoma cells using cell imaging. All results suggest that the new method is sensitive and promising for use in cancer diagnosis and treatment.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbono
/
Beta-Glucosidase
/
Carcinoma Hepatocelular
/
Pontos Quânticos
/
Inibidores de Glicosídeo Hidrolases
/
Neoplasias Hepáticas
Limite:
Humans
Idioma:
En
Revista:
Luminescence
Assunto da revista:
BIOFISICA
/
BIOQUIMICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China