TGF-ß1 sustains germ cell cyst reservoir via restraining follicle formation in the chicken.

ABSTRACT

The transforming growth factor ß (TGF-ß) superfamily members are important molecules that regulate many ovarian functions under normal physiological and pathological conditions. TGF-ß1 and its receptors are highly expressed in the ovarian cells of many species. However, the effect of TGF-ß1 on the capacity of the avian germ cell reservoir remains unknown. In this study, 5-day-old chicks were injected with TGF-ß1 (2.5, 12.5, and 62.5 µg/kg body weight) for 3 days to assess the effect of TGF-ß1 on early follicle development. Morphological analysis showed that treatment with TGF-ß1 (12.5 µg/kg) increased the number of germ cell cysts and reduced the number of primordial and growing follicles. The diameter and area of oocytes and follicles were decreased after TGF-ß1 treatment. Immunohistochemical staining of the proliferating cell nuclear antigen revealed that the ratios of the positive somatic and granulosa cells were decreased by 16.2% and 2.48%, respectively. Furthermore, more apoptotic cells were observed in the TGF-ß1 group than those of the control by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. In addition, we cultured the 5d chicken ovaries for 3 days in vitro and found that treatment with TGF-ß1 (10 ng/mL) manifested similar results as the in vivo experiment. However, the negative effect of TGF-ß1 on early ovary development was rescued by treatment with a TGF-ßR1 inhibitor SD208, resulting in increased expression of steroidogenic enzymes and cell cycle-regulating proteins. In conclusion, TGF-ß1 could maintain the germ cell reservoir by restraining follicle activation involving reduced cell proliferation and steroidogenic enzymes gene expression at the early stage of ovarian development.