Neuronal firing modulation by a membrane-targeted photoswitch.
Nat Nanotechnol
; 15(4): 296-306, 2020 04.
Article
em En
| MEDLINE
| ID: mdl-32015505
ABSTRACT
Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. In this respect, azobenzene-based photoswitches are promising nanoscale tools for neuronal photostimulation. Here we engineered a light-sensitive azobenzene compound (Ziapin2) that stably partitions into the plasma membrane and causes its thinning through trans-dimerization in the dark, resulting in an increased membrane capacitance at steady state. We demonstrated that in neurons loaded with the compound, millisecond pulses of visible light induce a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. These effects are persistent and can be evoked in vivo up to 7 days, proving the potential of Ziapin2 for the modulation of membrane capacitance in the millisecond timescale, without directly affecting ion channels or local temperature.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Azo
/
Potenciais de Ação
/
Membrana Celular
/
Hipocampo
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Nat Nanotechnol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Itália