Structure-guided reshaping of the acyl binding pocket of 'TesA thioesterase enhances octanoic acid production in E. coli.

Deng, Xi; Chen, Liuqing; Hei, Mohan; Liu, Tiangang; Feng, Yan; Yang, Guang-Yu

Deng, Xi; Chen, Liuqing; Hei, Mohan; Liu, Tiangang; Feng, Yan; Yang, Guang-Yu.

Afiliação

Deng X; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Chen L; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Hei M; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Liu T; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430072, China.
Feng Y; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: yfeng2009@sjtu.edu.cn.
Yang GY; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: yanggy@sjtu.edu.cn.

Metab Eng ; 61: 24-32, 2020 09.

Article em En | MEDLINE | ID: mdl-32339761

Assuntos

Caprilatos/metabolismo; Proteínas de Escherichia coli; Lisofosfolipase; Proteínas Periplásmicas; Sítios de Ligação; Escherichia coli/genética; Escherichia coli/metabolismo; Proteínas de Escherichia coli/química; Proteínas de Escherichia coli/genética; Proteínas de Escherichia coli/metabolismo; Lisofosfolipase/química; Lisofosfolipase/genética; Lisofosfolipase/metabolismo; Proteínas Periplásmicas/química; Proteínas Periplásmicas/genética; Proteínas Periplásmicas/metabolismo; Engenharia de Proteínas

Palavras-chave

Fatty acid; Octanoic acid; Saturation mutagenesis; Substrate selectivity; Thioesterase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Caprilatos / Proteínas de Escherichia coli / Proteínas Periplásmicas / Lisofosfolipase Idioma: En Revista: Metab Eng Assunto da revista: ENGENHARIA BIOMEDICA / METABOLISMO Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

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